Fusarium verticillioides mapping
The genetic map of Fusarium verticillioides(Xu et al, 1996) is based on the segregation of 142 restriction fragment length polymorphism (RFLP) markers, two auxotrophic genes (argl, nicl), mating type (matA+/matA-), female sterility (stel), spore-killer (Sk), and a gene governing the production of the mycotoxin fumonisin B1 (fuml) among 121 random ascospore progeny from a single cross. The map includes 12 linkage groups corresponding to the 12 chromosome-sized DNAs.
The plasmid clones containing the 142 RFLP markers were sequenced in both directions at the Broad. Both forward and reverse sequences were treated as independent markers, resulting in 286 marker sequences. 131 of these markers were located in our current assembly using BLASTN. You can download the read file (fv3markers.fasta.gz) for these 131 successfully mapped sequences and the detailed mapping information (fusarium_verticillioides_3_assembly_map.xml) from the Downloads page.
More than 99% of the assembly was anchored to the genetic map by locating the position of the sequence of these RFLP marker sequences within the current assembly using BlastN. You can view the genetic linkage group maps graphically using the links to the left. A poster sized version of all of the physical & genetic maps is also available.
The majority of unplaced markers on the map were caused by incomplete sequence data. However, 44 markers were rejected for discrepancies between forward and reverse reads of the marker or for alignment to a supercontig whose consensus mapping was to a different chromosome. Details about these rejected markers can be found in the individual chromosome map.