What's New
August 16, 2004
We are pleased to announce the publication of the following:
Jacob D. Jaffe, Nicole Stange-Thomann, Cherylyn Smith, David DeCaprio, Sheila Fisher, Jonathan Butler, Sarah Calvo, Tim Elkins, Michael G. FitzGerald, Nabil Hafez, Chinnappa D. Kodira, John Major, Shunguang Wang, Jane Wilkinson, Robert Nicol, Chad Nusbaum, Bruce Birren, Howard C. Berg, George M. Church 2004. The complete genome and proteome of Mycoplasma mobile. Genome Research 14: 1447-1461.ABSTRACT
Although often considered "minimal" organisms, mycoplasmas show a wide range of diversity with respect to host environment, phenotypic traits, and pathogenicity. Here we report the complete genomic sequence and proteogenomic map for the piscine mycoplasma Mycoplasma mobile, noted for its robust gliding motility. For the first time, proteomic data are used in the primary annotation of a new genome, providing validation of expression for many of the predicted proteins. Several novel features were discovered including a long repeating unit of DNA of 2435 bp present in five complete copies that are shown to code for nearly identical yet uniquely expressed proteins. M. mobile has among the lowest DNA GC contents (24.9%) and most reduced set of tRNAs of any organism yet reported (28). Numerous instances of tandem duplication as well as lateral gene transfer are evident in the genome. The multiple available complete genome sequences for other motile and immotile mycoplasmas enabled us to use comparative genomic and phylogenetic methods to suggest several candidate genes that might be involved in motility. The results of these analyses leave open the possibility that gliding motility might have arisen independently more than once in the mycoplasma lineage.
June 3rd, 2004
A complete finished sequence and annotation of Mycoplasma mobile is now available. This release includes:
- Putative gene set (635 genes)
- Visualization tools, available from Search and View Regions
- Feature annotations including:
Assembly Data
October 4, 2004 Assembly 1
Sequencing Facts
- Greater than 10X sequencing coverage of the genome
- Circular genome with a size of 777,079 bp
- G+C content 24.95%
