Overview

Gene Set Enrichment Analysis (GSEA) is a computational method that determines whether an a priori defined set of genes shows statistically
significant, concordant differences between two biological states
(e.g. phenotypes).

What’s New

A new release of the Molecular Signatures Database (MSigDB) is now available. The release includes new gene sets based on KEGG pathways, GO annotations, and the module map for cancer compiled by Segal et. al. (Nature Genetics 36, 1090 - 1098, 2004). If you have questions, please contact us at: gsea@broad.mit.edu.

Getting Started

A quick tutorial to get you up and running.

Tools and Information

Downloads: Implementations of GSEA plus additional resources to analyze, annotate and interpret enrichment results.

Molecular Signatures Database: A collection of gene sets for use with GSEA software and tools for exploring them.

Documentation: Information on the GSEA software, the GSEA algorithm.

Registration

Please register to download the GSEA software and view the MSigDB gene sets. After registering, you can log in at any time using your email address. Registration is free. Its only purpose is to help us track usage for reports to our funding agencies.

Contributors

GSEA is maintained by the GSEA team. Our thanks to our many contributors. Funded by: National Cancer Institute, National Institutes of Health, National Institute of General Medical Sciences.

Citing GSEA

To cite your use of the GSEA software, please reference Subramanian, Tamayo, et al. (2005, PNAS 102, 15545-15550) and Mootha, Lindgren, et al. (2003, Nat Genet 34, 267-273).