Targeted genomic rearrangements using CRISPR/Cas technology.

Nat Commun
Authors
Keywords
Abstract

Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangement between pairs of targeted loci, providing a highly tractable approach for the study of genomic rearrangements.

Year of Publication
2014
Journal
Nat Commun
Volume
5
Pages
3728
Date Published
2014 Apr 24
ISSN
2041-1723
URL
DOI
10.1038/ncomms4728
PubMed ID
24759083
PubMed Central ID
PMC4170920
Links
Grant list
R01 CA109038 / CA / NCI NIH HHS / United States
NCI R01CA109038 / CA / NCI NIH HHS / United States