RNAi Platform

RNA interference (RNAi) is emerging as a powerful tool for mammalian genetic screens. The mission of the Broad RNAi platform is to realize the enormous potential of RNAi to identify the genes underlying disease processes and to elucidate fundamental biological mechanisms. The RNAi platform grew out of The RNAi Consortium (TRC), a collaborative effort of six academic research institutions and five leading life sciences organizations. The RNAi platform and TRC work as an integrated team to develop the materials and technology to enable and enhance RNAi as a tool for mammalian genetic screening. The materials and knowledge generated by this team will be made available to the entire scientific community.

The RNAi platform is directed by David Root, a physical chemist with significant experience in cell-based screening and building lentiviral-based libraries.

The platform's major activities include:

• Library production
  Platform scientists are creating genome-scale libraries of RNAi reagents, targeting virtually all human and mouse genes, which are carried in lentiviral vectors that allow this library to be introduced into a wide range of cell types. They have also developed a streamlined production process for rapid expansion of the library.
  
  
• Library screening and validation
  Platform scientists are developing and optimizing methods for using the library in high-throughput screening. This has enabled the first RNAi genetic screens by lentiviral infection.
  
  Platform scientists are developing and optimizing methods for using the library in high-throughput screening. They have completed the first arrayed RNAi genetic screen by lentiviral infection.
  
  
• Library development
  New versions of the library are being developed, as well as new methods to screen large subsets of the existing library.
  
  
• Informatics
  Informatics scientists in the platform are developing archival and analytical tools necessary to ensure the utility of this library for all scientists.